General comments
Most candidates were well prepared and familiar with practical techniques. Areas of weaker understanding were concentration vs volume (noted in Q1(a)(iii), Q1(b)(ii), Q2(b)) and the hydrogencarbonate indicator (Q1(d)). Tables, graphs and observations were generally good. Improvement suggestions in Q1(b)(i) and planning in Q2(b) showed where more lab practice helps.
Table & headings
ModerateMost tables were suitable. Issues: vague heading “test-tube” instead of “glucose concentration”; some included units inside the body of the table, losing full credit.
Conclusion vs trend
ModerateMany stated a suitable conclusion; a significant number described the trend instead of linking back to the stated aim.
Independent variable
ModerateMost identified concentration of glucose. Some incorrectly gave volume (too vague since final volume was standardised).
Control variable (with detail)
ModerateTemperature was common. Some answers lacked detail—e.g., “yeast kept constant” is insufficient; “volume of yeast kept constant” would gain credit.
Why stir the yeast suspension?
DifficultFew correct explanations. Step 5 had no glucose; stirring was to evenly distribute yeast cells, not to increase activity, and yeast does not “dissolve.”
Improvements to the investigation
DifficultMany suggested changes to steps that didn’t need improvement or proposed different investigations instead. Repetition was the most common valid idea.
Make 5.0 cm?3; of 0.25% glucose from 0.50%
DifficultAlthough some described it well, many couldn’t determine the correct dilution or make the specified volume.
Benedict’s test for glucose
EasyAlmost all knew the reagent and that heating is required. A few forgot “hot” water-bath or confused iodine–starch test.
Apparatus for collecting CO?
ModerateGas syringe was the common correct choice. “Measuring cylinder” alone needed displacement/over-water detail.
What is an anomalous result?
EasyBest description: a measurement that doesn’t fit the expected pattern. A few thought it means “inaccurate.”
Means with anomalies
EasyMost knew to exclude the anomalous value. A few then divided by three instead of two.
Compare mean volumes (25 °C vs 35 °C)
ModerateSome produced detailed comparisons and manipulated data correctly; explanations were not required.
Graph quality & key
ModerateMostly neat with labelled axes/plots/lines. Issues: extrapolating to origin, zero points missing, wrong time unit, cramped scale, wrong axis for independent variable, dashed-line keys (prefer markers).
Estimate time from graph
ModerateMost estimated correctly and indicated on graph; some marks were too small to show how the value was obtained.
Hydrogencarbonate indicator colour change
DifficultFew knew the correct colour change when CO? is bubbled through the solution.
Drawing cell X (bio drawing conventions)
EasyNearly all drew the single labelled cell with sharp pencil and continuous lines, no shading; many careful, detailed observations.
Actual length (3 s.f.)
EasyMany correct calculations with answers to three significant figures.
Plan investigation: NaCl concentration & osmosis
DifficultMany strong plans using different NaCl concentrations; some confused concentration with volume or failed to standardise volumes. Common valid methods: measure mass/length before & after immersion for a set time. Frequent mistakes: measuring volume of solution before/after immersion, dialysis tubing with no plant tissue, confusing osmosis with transpiration (potometer/dye). Safety: goggles/gloves often unnecessary—most appropriate is safe knife use (cut away from body/on a hard flat surface).
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